English translation for "expression cloning"

表达克隆[通过进行基因表达而克隆目的基因的方法
表达克隆化

Related Translations:

overlapping clones:  重叠克隆

expression simplification:  表式简化

expression equipment:  压榨设备216

boolean expressions:  布尔表达式

expression element:  表式元素

creative expression:  创造性表达

graphic expression:  图示

infix expression:  插入表达式

expression cystourethrogram:  压迫性膀胱尿道造影片

expression system:  表达系统

Example Sentences:

	1.	In our study we have cloned the osd gene from s . typhimurium by pcr , characterized the gene product and used this gene to construct asd + expression cloning vectors ptrc99a - asd 再将hpylori尿素酶b亚单位基因与尿素酶b和热休克蛋白a融合基因分别克隆入ptrc99a一asd质粒的多克隆位点之内。
	2.	Conclusion constructed the high - level expression clone of echistatin in e . coli . the expression of recombinant protein is higher , it make the further study of echistatin feasible 结论成功构建了echistatin的原核高效表达克隆，表达量高于现有国内外研究水平，为echistatin功能及相关疾病的研究奠定了基础。
	3.	In the first part , the focus is to find the receptor molecules directly by screening two cdna libraries with a recombinant construct prpap or as an alternative , to find an enriched area in the embryo brains and construct libraries from this brain region and perform the expression cloning as above 方法： （ ）以融合蛋白prpap通过瞬时表达克隆法筛选两个cdna文库，或者通过与胚胎脑的结合实验筛选有丰富结合蛋白的脑区，以图构建cdna文库并进行表达克隆的筛选。
	4.	Objective : construct high - level expression system of echistatin in e . coli methods : obtain amino - acid sequence of echistatin from genebank database . considering the bias of usage of 61 available aminoacid codons in e . coli , design the coding sequence of echistatin , synthesize the dna sequence chemically , get single copy coding gene and repeated two copy coding gene of echistatin . insert the sequence into expression vector pbv220 , and more , we construct fusion expression clone of echistatin with pcr , identify the recombinant vector by dna sequencing 目的构建蛇毒锯鳞蝰素( echistatin )的原核高效表达体系方法由genebank数据库检索蛇毒锯鳞蝰素( echistatin )的氨基酸序列，结合大肠杆菌蛋白质合成体系对氨基酸密码子使用的偏爱性，设计了echistatin编码基因，体外人工合成编码基因dna片段，通过适当的限制性内切酶位点插入表达载体pbv220 ，分别构建了echistatin的单拷贝表达克隆、双拷贝串联表达克隆；进一步通过pcr技术构建echistatin的融合表达基因克隆。

Similar Words：

"expression as bound or length" English translation, "expression atom" English translation, "expression behaviour" English translation, "expression browser" English translation, "expression classifications" English translation, "expression cloning phenotype cloning" English translation, "expression compute point analysis" English translation, "expression constant" English translation, "expression containing conditions" English translation, "expression continuation" English translation